ucture using solid-state NMR
نویسندگان
چکیده
An overview of solid-state NMR structural studies on various silk forms and analogs conducted by the authors' research groups is presented. The well-studied silkworm and spider silks together with related silk peptides have a mixture of secondary structures including b-sheet, b-turn, helix and random coil that are difficult to analyze by X-ray diffraction and electron microscopy but conveniently investigated by solid-state NMR. Several newly developed solid-state NMR techniques and stable isotope labeling approaches of the silks were effectively used to characterize silk structure. The techniques discussed provide not only information on the secondary structure, but also on the hydrogen-bonding interactions present in the silks. Structural studies on other types of silk, silk peptide mimics and recombinant silk proteins are also discussed.
منابع مشابه
A software framework for analysing solid-state MAS NMR data
Solid-state magic-angle-spinning (MAS) NMR of proteins has undergone many rapid methodological developments in recent years, enabling detailed studies of protein structure, function and dynamics. Software development, however, has not kept pace with these advances and data analysis is mostly performed using tools developed for solution NMR which do not directly address solid-state specific issu...
متن کاملRapid acquisition of wideline MAS solid-state NMR spectra with fast MAS, proton detection, and dipolar HMQC pulse sequences.
The solid-state NMR spectra of many NMR active elements are often extremely broad due to the presence of chemical shift anisotropy (CSA) and/or the quadrupolar interaction (for nuclei with spin I > 1/2). These NMR interactions often give rise to wideline solid-state NMR spectra which can span hundreds of kHz or several MHz. Here we demonstrate that by using fast MAS, proton detection and dipola...
متن کاملSimultaneous use of solution, solid-state NMR and X-ray crystallography to study the conformational landscape of the Crh protein during oligomerization and crystallization
We explore, using the Crh protein dimer as a model, how information from solution NMR, solid-state NMR and X-ray crystallography can be combined using structural bioinformatics methods, in order to get insights into the transition from solution to crystal. Using solid-state NMR chemical shifts, we filtered intra-monomer NMR distance restraints in order to keep only the restraints valid in the s...
متن کاملApplication of Spin Counting to the Solid-State 31P NMR Analysis of Pasture Soils with Varying Phosphorus Content
100% natural isotopic abundance, and has a nuclear spin of 1/2 that ensures relatively easy detection and Solid-state 31P NMR spectroscopy has the potential to identify spectral interpretation. Two different modes of 31P NMR forms of soil P without the need for extractions or pretreatment. We used both cross polarization (CP) and direct polarization or Bloch spectroscopy—solution and solid-stat...
متن کامل6 SCIENTIFIC HIGHLIGHT OF THE MONTH : ” First principles calculation of Solid - State NMR parameters ” First principles calculation of Solid - State NMR parameters
The past decade has seen significant advances in the technique of nuclear magnetic resonance as applied to condensed phase systems. This progress has been driven by the development of sophisticated radio-frequency pulse sequences to manipulate nuclear spins, and by the availability of high-field spectrometers. During this period it has become possible to predict the major NMR observables using ...
متن کامل